Other than the phages Typhi and Paratyphi, many Salmonella species can cause non typhoid salmonellosis. NTS organisms are frequent food pollutants. Poultry alone accounts for up to 50% of salmonellosis outbreaks and is also carried by wild creatures, rodents, wildlife, and reptiles, usually without any symptoms of illness. Poultry meat, eggs, milk, vegetables, and water have also been reported as important sources for epidemics of human Salmonella infection. These illnesses are among the most prevalent food-borne zoonotic infections in both industrialized and developing nations; however the incidence varies by nation. NTS infection in poultry has grown in Egypt over the past few years. Antibiotic overuse in zoonotic illnesses has resulted in the emergence of resistant bacteria that can be transmissible to humans in three different ways. The study comprises stool samples and clinical information from a significant clinic in Minia, Egypt, where 500 stool samples from gastroenteritis patients were gathered. All participants failed the essential negative widal test to be included in the study. Standard microbiological testing and serological tests were used to evaluate the stool samples and identify non-typhoid Salmonella. A panel of 11 discs representing 11 different antibiotic groups, including tetracycline, chloramphenicol, ampicillin, streptomycin, nalidixic acid, cefotaxime, and sulphamethoxazole-trimethoprim, were used to assess the antimicrobial susceptibility of the samples. The Medical Laboratory Standard Institute's guidelines were used to interpret the results. MDR is defined as isolates that are resistant to at least one component of three distinct antimicrobial classes. Using the double disc synergy test, extended-spectrum lactamase production was found. The tetracycline resistance-related gene and the enzyme-coding gene for chloramphenicol acetyl transferees were amplified by PCR. Shown in agarose gel with the UV Trans illuminator performed an antimicrobial sensitivity test the majority of isolates were resistant to ampicillin, tetracycline, trimethoprim-sulphamethoxazole, and only 27.3% of isolates were resistant to chloramphenicol. This was a good result because chloramphenicol may once again be an effective drug for treating NTS infections, particularly in developing nations. However, the majority of isolates were amikacin and susceptible and only 18% of isolates produced ESBLs, whereas 81.8% were multidrug resistant (MDR). In 64.7% and 50%, respectively, of the samples sensitive to tetracycline and chloramphenicol, and cat genes were found. Hese genes can provide a hint as to the Tetracycline and chloramphenicol resistance mechanisms in resistant isolates. This showed a significant amount of MDR non-typhoid Salmonella. The results of screen studies like this could be utilized as future medical references and in developing preventive steps to control NTS illnesses in this area. Deeper investigation into the molecular mechanisms of resistance